Composition and Domains

The ZNF280D protein is 979 amino acids in length.^[11] The protein contains a domain of unknown function (DUF4195) spanning from amino acid 45 to amino acid 230.^[11] DUF4195 (pfam13826) is a family that is found at the N-terminus of metazoan proteins that carry PHD-like zinc-finger domains; the function is unknown.^[12] ZNF280D protein also contains five highly conserved Cys2His2-type zinc finger domains.^[13] Zinc fingers have the ability to bind DNA, which supports the speculative role of ZNF280D as a transcription factor.^[14] The protein has a weight of approximately 109.3 kdal.^[15] Charge cluster analysis reveals a negative charge cluster near the N-terminus from amino acids 16-43.^[15] Charge clusters are associated with functional domains of cellular transcription factors, providing further support for ZNF280D as a possible transcription factor.^[16]

Interactions

ZNF280D has been experimentally determined to interact with CBX5 and CBX3 proteins.^[17] These proteins both play a role in the formation of heterochromatin, which presents a possible functional role of ZNF280D as a transcriptional repressor.^[18][19]

SNPs

There are a number of SNPs that have been observed in the human population.^[20] The image below lists some of the most frequently occurring.

mRNA Level

A number of transcription factors are predicted to bind to the predicted promoter region.^[21]

Protein Level

ZNF280D protein contains 66 serine, 17 threonine, and 6 tyrosine residues all of which are potential phosphorylation sites.^[22]

The glycine residue at position 2 is a probable candidate for N-terminal acetylation.^[23] There are seven probable sumoylation sites.^[24]