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• Roxie McTerrier (voiced by Diana Kaarina) is a hyper, optimistic and loyal Boston Terrier.^[4] She cares a lot about her friends, but she can be very naive at times. She likes eating food and having fun.
• Jade Catkin (voiced by Ingrid Nilson) is a sarcastic and gothic bombay cat^[4] who is Roxie's roommate ever since the latter destroyed the floor in her old home. She is also Roxie's best friend but dislikes admitting it. Her favorite thing to do is sleep.
• Trip Hamston (voiced by Travis Turner, who, like the character, is a rapper) is an adventurous and confident but sometimes goofy hamster.^[4] He is also a rapper.
• Quincy Goatee (voiced by Kyle Rideout) is an easily frightened fainting goat who faints easily.^[4] He is Trip's best friend and roommate. In the episode "Pitch Im-Purr-Fect" it is revealed that he isn't good at singing but he is a good tap dancer.
• Edie Von Keet (speaking voice by Lili Beaudoin and singing voice provided by Diana Kaarina) is a fancy and dramatic parakeet who is a talented actress. She always tends to be greedy for the spotlight, which is a reflection of her 14-year-old owner.^[4]
• Bev Gilturtle (voiced by Rhona Rees) is an energetic blue box turtle.^[4] She tries her hardest to entertain the other pets. She likes trying out various kinds of activities.

Recurring

• Savannah Cheetaby (voiced by Bethany Brown) is a very famous cheetah pop star in Paw-Tucket.
• Petula Woolwright (voiced by Diana Kaarina) is a purple sheep who is the main antagonist of the series and the leader of "the snobs".
• Sweetie Pom-Pom (voiced by Brittney Wilson) is a tan pomeranian who is one of Petula's sidekicks.
• Gavin Chamelle (voiced by Alessandro Juliani) is a chameleon in Petula's gang. Juliani also voices Mayor Perrito, a Chihuahua who acts as the self-appointed mayor of Paw-Tucket due to his name being revealed as Mayor.
• Mitchell Snailford (voiced by Ian Hanlin) is a dimwitted brown snail and another one of Petula's sidekicks.
• Mister Yut (voiced by Vincent Tong) is a red Siamese fighting fish responsible for managing the commerce in Paw-Tucket. Tong also voices Scoot Raccoonerson, a sneaky raccoon who thinks that trash is valuable, and Austin Goldenpup, an obedient Golden Retriever in the human world, whom Roxie has a crush on and who works for Mister Yut at the Littlest Pet Shop.
• Captain Gilturtle (voiced by Brian Drummond) is a blue sea turtle, Bev's uncle, and head captain of the LPS cruise ship.
• Manny Mouser (voiced by Ryan Beil) is a highly intelligent yellow mouse, an inventor.
• Carmilla Wingbat (voiced by Lanie McAuley) is an incredibly shy fruit bat who prefers to stay in the shadows. She has an incredible singing voice and acting skills. She works as a crew member, and later, actress at Paw-Tucket theater. She also has the ability to use echolocation.
• Pearl (voiced by Shannon Chan-Kent) is a salon cat worker from the shaking dry salon.

"Littlest Pet Shop" shorts

"Late Night Bev" shorts