Adults

• Gus (voiced by Ed Gilbert) — Kissyfur's widowed father, who owns a Paddlecab company, taxiing animals from one side of the swamp to the other. He can be a bit goofy at times, but is a very good father. He's the only one of all the swamp parents who can take on both alligators, Floyd and Jolene, and run them off.
• Miss Emmy Lou (voiced by Russi Taylor) — a blue bear who wears a flower behind one ear. She's the schoolteacher in the swamp and has a Southern accent. She is also an excellent cook and has a sister named Jenny Lou, a niece named Donna and a cousin named Ernie. She is sweet on Gus and serves as his love interest.
• Charles (voiced by Lennie Weinrib) — a warthog and Lennie's stubborn father, Charles thinks he has everything figured out most of the time, but is usually more brawn than brains. He has several conflicts with Gus. He is strong enough to take on Jolene, but not Floyd. He is one of the only three adults to be captured and almost eaten by the gators, along with the Cackle Sisters.
• Howie (voiced by Frank Welker) – a mocking bird who can throw his voice and can mimic anything and everyone. This talent often gets him into trouble.
• Uncle Shelby (voiced by Frank Welker) - a wise turtle who is the elder of the swamp.
• The Cackle Sisters (portrayed by Russi Taylor and Frank Welker) — two chicken sisters named Bessie and Claudette. Bessie talks and is very prim and proper, while Claudette just clucks, usually agreeing with whatever her sister says. They are usually seen keeping watch for Floyd and Jolene on a large, floating buoy and are quick to ring a bell whenever they catch sight of them. They are two of the only three adults to be captured and almost eaten by the gators, along with Charles.
• Floyd (voiced by Stu Rosen) — an alligator who, along with His sister, Jolene, is always hatching a plan to capture the swamp cubs so they can eat them for dinner (though if the opportunity arises, they may sometimes go after the adults, as well). He often makes dimwitted remarks.
• Jolene (voiced by Terence McGovern) — a hot-tempered alligator who wears a red wig. She and Floyd are always trying to capture the swamp cubs so they can eat them for dinner (though if the opportunity arises, they may sometimes go after the adults, as well). She would be considered the brains between the two, but not by much. She has a low tolerance for Floyd's dimwittedness, which usually results in her smacking him with her wig.
• Flo (voiced by Marilyn Lightstone) — a smug buzzard. Her companion is a snake named Reggie.

Swamp cubs

• Kissyfur (voiced by R.J. Williams and later Max Meier) — Gus's son, the leader of the swamp cubs, and the title character of the series. He and his father used to work in a circus, along with Kissyfur's mother, who died in a performance accident. After the circus train, they were on crashed, Kissyfur and his father ran into Paddlecab County, and that's where they have been living since. He is an eight-year-old bear cub who loves to pretend and occasionally gets into trouble with the rest of the cubs. His name is a malapropism of the name Christopher as Phil Mendez named the character after his son.
• Stuckey (voiced by Stu Rosen) — an indigo porcupine who is very gloomy. He talks slowly and is the quiet one of the group. He is also Duane's best friend and is the only swamp cub whose family is not seen. According to the revised writer's bible, Stuckey was an orphan found on a road, and adopted by an unseen family of possums. He is also the only swamp cub not to speak in the pilot.
• Beehonie (voiced by Russi Taylor) — a white bunny who has a crush on Kissyfur, on account of the fact that she is the same age as he is. She is the only female swamp cub and tends to act as the voice of reason at times.
• Duane (voiced by Neil Ross) — an eleven-year-old pig who loves to clean, similar to Felix Unger of The Odd Couple, with the manners and attitude of Truman Capote. He is Stuckey's best friend.
• Toot (voiced by Russi Taylor) — a three-year-old beaver, Toot is the youngest of the swamp cubs. He looks up to and idolizes Kissyfur. He is Kissyfur's best friend. His nose changes from pink to black in season two.
• Lennie (voiced by Lennie Weinrib) — Charles's son, Lennie is ten years old and was the leader of the swamp cubs prior to Kissyfur's arrival (after which leadership transferred to him without much explanation). He enjoys being bossy and pushing the other cubs around, although despite this, he does like and care about his friends. He often refers to Kissyfur as "Sissyface".
• Ralph (voiced by Susan Silo) — a young packrat, the same age as Kissyfur. sort of the rebel of the cubs.
• Flip (voiced by Tress MacNeille) — a tricky chameleon that can change colors. In season 1, he had a red color for the top of His body, yellow with red spots in the middle, and blue with a light-blue spot and in between. In season 2, he has a green body, with a yellow stomach, but can still change colors.
• Donna (voice by Russi Taylor) — Miss Emmy Lou's niece. Her only appearance is in the second special, "The Birds and the Bears".

Season 1 (1985–1986)

The first four episodes were primetime specials aired between 1985 and 1986.^[6]

Season 2 (1988)

Additional voices

• Brian Cummings
• Jeannie Elias
• Ron Feinberg
• Linda Gary
• Barbara Goodson
• Michael Horton
• Mona Marshall
• John Stephenson
• Jill Wayne