As they wait for news on Kenny, who is being treated for wounds he received after being shot by his old friend, Curtis Williams, an Army veteran turned hired assassin, while assisting agents Maggie Bell and OA Zidan of the FBI's New York field office on a case which started with the murder of a woman on federal property and led to the uncovering of a sex trafficking ring during the FBI Season 4 premiere episode, "All That Glitters", the Fugitive Task Force are assigned to hunt down a suspect believed to be responsible for the murder of billionaire Nathan Tate which triggers a gunfight in downtown New York City that ends with both the suspect and an NYPD officer dead and has the team meeting Kristin Gaines, an agent from the FBI's Miami field office who has been investigating the suspect as well as his movements. Jess also learns that he and Kenny have been cleared of the shooting that occurred at Jess's house which also reveals that Kenny shot dead Hugh Hill, the ex-husband of Jess's girlfriend, Sarah Allen. With assistant from OA who temporarily joins the team and Kristin, they race to apprehend Colin Kent and his associates, only to discover that Kent has not only fled the mansion he was staying in via an airplane and is heading to an unknown location but that he now has information concerning the identities of those involved in the sex trafficking ring.

As Hana rejoins the team while struggling to adjust the new changes in her life, the team find themselves investigating a deadly fire at an arcade in Pennsylvania, leaving two dead and one severely injured. Gradually they discover that Fred Duncan, the father of the arcade's manager, hired two people to torch the place to gain insurance money in order to pay his son's gambling debt. Said two people are later found dead alongside two more victims, with the latter of which having a connection to Angelo Carpenter, who served 35 years in prison for a rape and murder he never committed and is now seeking revenge against Fred Duncan, who was the real culprit, Fred's father, Fred Duncan Senior having paid those involved to frame Angelo while protecting his son, Fred Junior. He kidnaps his granddaughter, Abby and returns them to the Duncan residence to re-enact said events. The Task Force surround the residence, rescuing Fred and Abby but Carpenter attempts to escape, only to be shot dead by Ortiz while Abby is reunited with her mother and Fred Junior is taken into custody. Hana connects with Ortiz, deciding to go to the bar with him.

• Tim DeKay (Angelo Carpenter) is credited as a Special Guest Star.

The team investigate when a prized Kentucky race horse is stolen with its groomer being taken hostage. They also learn that two murders at a country golf club and of the horse transport's driver came in addition as collateral damage. The groomer, Tessa Sanders, manages to convince Willy Burke, Solomon Pensky's accomplice, to let her go and work together to get the money demanded from the horse owner, Grace Roland, however she ditches him when the FBI is caught up on them. Initially Roland stonewalls the Task Force citing the demands of no law enforcement involvement, but is convinced by Barnes and Gaines. With the horse's return, Roland decides to let Sanders get away with the money, expressing that she earned it for her work with the horse. Byron, Jess's father returns to celebrate the holidays with Jess and Tali, while Jess also worries about the possibility of Tali getting accepted into a boarding school in Canada.

• Terry O'Quinn (Byron LaCroix) is credited as a Special Guest Star.

A trip to the mall for some holiday shopping turns deadly for Jess and Barnes when they along with the other shoppers find themselves caught in the middle of a shooting with the exits being rigged with so that no-one can escape. Gibson, Ortiz and Gaines are notified and in turn is the NYPD, who works with them to defuse and locate the bombs and finding the mastermind behind it. On the inside, LaCroix and Barnes secures the former's family along with other shoppers in a staff room and work with former cop Linwood to disarm the shooters and find more survivors. A total of five bombs are discovered, but the fifth one remains of radar for the outside team until Ortiz discovers a timer at the mastermind, Rob Murphy's house. Linwood risks his life to save LaCroix and Barnes from the bomb while Byron assists Gaines and SWAT entry through the roof. With all bombs defused and shoppers evacuated, Charlotte, who was also trapped in the mall, goes into early labor and gives birth to a baby boy. Linwood is reunited with his son and LaCroix finds himself supporting Tali who's going to boarding school in Canada, with her application having been accepted.

• Terry O'Quinn (Byron LaCroix) is credited as a Special Guest Star.

The Fugitive Task Force takes the case of Binh Dao, who utilised napalm bombs in a casino, injuring two and killing one. Initially they speculate his motives are political, but this changes when he targets multiple people who had a role in his upbringing, including the mother of his childhood friend. The Task Force learns from his stepfather that he shot his marine roommate for racist remarks, which was reported as an accident. They later discover that the stepfather gambled money away at the casino targeted earlier, which was meant for the mother's back surgery. Binh further targets his mother's former employer and heads to the lab that his mother once interviewed for because of her PhD engineering degrees. His ultimate motive is deduced to be standing up against the ones he thinks wronged his mother. The Task Force and lab security manage to stop Binh on their property and talk him into surrendering with his mother's help. LaCroix and Sarah see Tali off as she leaves for boarding school and share a movie night with his sister.

• This episode marks the final appearance of Yaya Gosselin (Tali Skye LaCroix).

LaCroix and the Task Force search for Harley Ross, the abusive boyfriend of Lucy Carver, who killed Dr. Darien Johnson and his family in the search for her. They learn that Lucy sought sanctuary and protection from a covert website specialising in domestic abuse victims, and Ross continues his killing spree of people who would have insight to her whereabouts. They manage to save her handler, Alicia Dutton, while also learning that Lucy had a daughter, which triggered Ross. With intel from her, Ross goes to the ER to find their daughter who needs a transplant due to her having leukaemia, and sneaks out when the FBI surrounds him. LaCroix finds a bruised Lucy in the parking lot and shields her when Ross re-exits and begins shooting at the two. The rest of the FBI team arrive, shooting Ross dead while learning that Jess has been fatally wounded after Ross shot him in the neck. The ER personnel at the hospital attempt to treat Jess but he presumably dies off screen, devastating the entire team. Barnes and Gibson informs Sarah of LaCroix's passing, putting their travel plans on indefinite hold. Together, Sarah and Byron go to Canada to inform Tali of her father's death.

• This episode marks the final appearance of Julian McMahon (Jess LaCroix) while Terry O'Quinn (Byron LaCroix) is credited as a Special Guest Star.

Struggling to come to terms with Jess's death, Barnes, Hana, Ortiz and Gaines are given the assignment of tracking down a member of the incel community, Erik Perwin, who has begun embarking on a murder spree, targeting the people he believes never even gave him a chance. The incel community is in an uproar and another member, Jericho Brown, shoots up a saloon and seeks to work together with Perwin. They target a fashion show, where Perwin takes his idol model Chance Robbins hostage while Brown is shot when he brandishes his weapon. Gaines distracts Perwin, until Robbins breaks free himself and Ortiz subsequently tackles Perwin. Isobel Castille checks in on the team upon giving them the assignment, and the team decides to help Sarah finish the painted walls in her and Jess' home and share a meal with her and Byron.

• Terry O'Quinn (Byron LaCroix) is credited as a Special Guest Star.

When married couple Marianne and Will Conway, who grow illegal marijuana, kill their employees, the team head to Vermont to investigate and find the couple. Along the way, they learn that their operational application for marijuana has been rejected and that much of their personal information remain cryptic. Gibson and Gaines are trapped in their marijuana cellar when the couple returns home for seeds used as insurance, but Barnes frees them. From analogue records, they learn that Marianne is the daughter of Russian mobster Vasily Matrushok, whom they suspect ordered their daughter's kidnapping, only to learn that he retired longtime ago and left the mob's leadership to Marianne's sister Laura. Marianne and Will meet her at a secret marijuana site that Will set up, and she hands over their daughter in return for their seeds, but quickly goes back on her word just as the Task Force arrive with the resulting gunfight seeing Will and Laura being shot dead and Marianne being arrested while the couple's daughter is rescued. Barnes contemplates seeking maternity leave to be more present in her newborn son's life, which Castille accepts just as the CID's announcement of a new Task Force leader comes through.

• Alana De La Garza (Isobel Castille) is credited as a Special Guest Star.

The team are introduced to their new leader, Remy Scott who's returned to live in New York, just as they begin to investigate the supposed abduction of teenage girl Lillie Jones. They soon learn that she went voluntarily along with her troubled boyfriend Jeremy Hamm as both their parents are found dead and later also a private investigator. Both Hamm and Jones have ties to the Covenant church and Intentions rehab facility, with the former in practise being a cult. When the pastor is found dead, they suspect his wife killed him as they learn from an undercover IRS agent that the church was suspected to be involved in tax evasion. Lillie is also revealed to be pregnant and scheduled to be married to Jeremy in a ceremony held by one of the rehab center's leaders Ken Clymer. On scene, Hamm is found dead and they track a rental vehicle to a motel, where Pat Mitchell, the pastor's wife, is arrested and Lillie saved. Scott invites the team out to throw axes and drink, and also shares the story of his brother Mikey.

• This episode marks the debut appearance of Dylan McDermott (Remy Scott)

In 1989, a notorious gangster is killed at a drive-by fast food chain by hitman Jackie "The Fox" Bianchi. In 2022, a mother and her two young sons are killed in a similar fashion at the same location. Shortly after, three people are killed at a funeral, again replicating a hit perpetrated by Bianchi. The Task Force enlist the help of Terry Daniels, the former lead investigator from Organised Crime in the case against Bianchi, to utilise inner mafia sources to get ahead of the copy cat, Joey Messina, who idolises Bianchi. Scott attempts to convince judge April Brooks to sign off on a warrant to retrieve info from Bianchi's podcast despite him being presumed dead in 1992. The application is denied, but the team deduce that Bianchi is alive and resides in Las Vegas based on a cooking video. Scott's ex-wife Carmen from the LVMPD aids in the raid and arrest of Bianchi. Brooks signs of on a revised deal for Bianchi's assistance to lure out Messina. Messina changes meeting locations, but Bianchi utilises him as a means to escape, but is arrested and Messina is gunned down after refusing to surrender. With encouragement from Carmen, Scott decides to seek out Brooks, whom he has developed a small fascination for.

• Wendy Moniz, as Judge Brooks, begins a short (4 episodes) run as a love interest for the character of Remy Scott.

When Cassandra Mason, a female con artist pretending to be a personal trainer murders a wealthy couple from the East Hamptons, she goes on the run and murders the ones she thinks could expose her past. The Task Force struggle to pinpoint her real identity, linking Jenny Bentz to a private school Treetops, who seemingly drowned at the school lake. Accounts from her mother confirms that Mason stole Bentz's identity, and her real name is revealed to be Dehlia Holby. She tries to borrow money from her sister, but is furious when the amount is not enough, and takes her and her mother hostage. Through the Treetops principal's brother, a lawyer who helped settle a case against him about Dehlia's relationship with the former. It's because of this Dehlia has been doing what she has in order to escape her past. Scott talks her down and shoots her gun to disarm her. Ortiz is asked by Gibson's half brother George to lend him money to pay back a drug deal debt. Having considered his options, Ortiz ensures that George does pay him back by having him work at his local gym.

• This episode marks the final appearance of Miguel Gomez (Ivan Ortiz)