Bernardini is a family name of Italian origin.

As of 2014, 72.1% of all known bearers of the surname Bernardini were residents of Italy (frequency 1:2,561), 7.8% of France (1:25,728), 7.5% of Brazil (1:82,665), 5.3% of the United States (1:204,559) and 3.9% of Argentina (1:33,341).

In Italy, the frequency of the surname was higher than national average (1:2,561) in the following regions:^[1]

1. Tuscany (1:525)
2. Umbria (1:660)
3. Lazio (1:786)
4. Liguria (1:1,074)
5. Marche (1:1,267)

• Alain Bernardini, band's singer I Muvrini
• Albino Bernardini (1917–2015), Italian writer and pedagogue
• Adriano Bernardini (born 1942), Italian Roman Catholic prelate of the Catholic and former diplomat of the Holy See
• Alessandro Bernardini (born 1987), Italian footballer
• Antonino Bernardini (born 1974), Italian footballer
• Carlo Bernardini (artist) (born 1966), Italian artist
• Carlo Bernardini (politician) (1930–2018), Italian physicist and politician
• Charles Bernardini, alderman of Chicago's 43rd Ward from 1993 until 1999
• Dean Bernardini (born 1973), American bass guitarist and backing vocalist
• Domenico Antonio Bernardini (1647–1723), Roman Catholic Bishop of Mileto and later Bishop of Castellaneta
• Ernani Bernardini (1911–2006), big-band musician turned politician in Los Angeles
• Filippo Bernardini (1884–1954), Italian Roman Catholic Archbishop
• Fulvio Bernardini (1905–1984), Italian footballer and coach
• Jean François Bernardini, band's singer I Muvrini
• Laurentius Bernardini (17th century), Roman Catholic Titular Bishop of Coronea
• Marcello Bernardini (died 1799), Italian composer and librettist
• Mariano Bernardini (born 1998), Italian football player
• Micheline Bernardini (born 1927), French dancer
• Rita Bernardini (born 1952), Italian politician
• Roberto Bernardini (born 1944), Italian professional golfer
• Severino Bernardini (born 1966), Italian long-distance runner
• Simone Bernardini (born 1991), Italian racing cyclist
• Tiago Bernardini (born 1979), Brazilian footballer