The year 1535 in science and technology included a number of events, some of which are listed here.

• Jacopo Berengario da Carpi publishes Anatomia Carpi, the first anatomical text with illustrations, in Bologna.^[1]

• Alter Botanischer Garten Tübingen established by Leonhart Fuchs.

• March 10 – Fray Tomás de Berlanga discovers the Galápagos Islands when blown off course en route to Peru.

• June 21 – Leonhard Rauwolf, German physician and botanist (died 1596)
• Georg Bartisch, German physician and ophthalmologist (died 1607)^[2]
• William Butler, English physician (died 1617)
• Cornelius Gemma, Flemish physician and astronomer (died 1578)
• approx. date – Giambattista della Porta, Italian physician (died 1615)

• February 18 – Heinrich Cornelius Agrippa, German alchemist (born 1486)
• March 26 − Georg Tannstetter, Austrian physician and geographer (born 1482)