Promoted teams

These four teams were promoted from Druha Liha at the start of the season:

Relegated teams

Two teams were relegated from the Ukrainian Premier League 2005–06 season after finishing on the bottom of the competition:

• FC Volyn Lutsk : 15th place (Returning after four seasons)
• FC Zakarpattia Uzhhorod : 16th place (Returning after two seasons)

Renamed/relocated teams

• On May 23, 2006, the PFL Bureau of Ukraine acknowledged that FC Lviv will replace FC Hazovyk-Skala Stryi as its successor. The club was moved from Stryi to Lviv.
• On December 19, 2006 FC Dynamo-Ihroservis Simferopol changed to FC Ihroservis Simferopol.^[1]

Spartak Sumy

On November 28, 2006 PFL Bureau has excluded FC Spartak Sumy from competitions for the second no show. Due to the fact that team has physically played less than 50% of calendar games (17 games), all its season record was stripped.^[3] Upon withdrawal it had 1-0-18 record and 4-49 goals difference.

Borysfen Boryspil

On March 16, 2007 FC Borysfen Boryspil was excluded from competitions for systematic violation of statute and regulations of FFU and PFL, failure to comply with their organs of administration and implementation football justice as well as failure to fulfill terms of contracts with players and financial obligations to FFU and PFL. In the rest of games were nominated technical losses to the team and its opponents - technical victory.^[4]

Podillia Khmelnytskyi

In the second half of the season Podillia Khmelnytskyi moved from Khmelnytskyi to Krasyliv. At the same time for the amateur championship registered a new club from Khmelnytskyi, FC Podillia-Khmelnytskyi Khmelnytskyi. So, since spring 2007 there were two clubs Podillia, one at professional level playing in Krasyliv and other - amateur playing in Khmelnytskyi. One of the reasons was the prior merger of Podillia with FC Krasyliv back in 2004. At the end of 2006–07 season Podillia Krasyliv changed back to FC Krasyliv and was scheduled to play in the 2007-08 Ukrainian Second League, but withdrew just before the start of season.

Instead of Krasyliv to the Ukrainian Second League applied the newly revived FC Podillia-Khmelnytskyi Khmelnytskyi. In such manner there was created a club's continium.

Spartak Ivano-Frankivsk

FC Spartak Ivano-Frankivsk was relegated to the Ukrainian Second League, while another team form local university FC Fakel Ivano-Frankivsk obtain promotion to the Ukrainian First League. The city municipality on whose balance ended up both clubs decided to keep one and dissolve another one. So, in the 2007-08 Ukrainian Second League season there were no clubs playing from Ivano-Frankivsk, while a new team FSC Prykarpattia Ivano-Frankivsk was created in place of Fakel to participate in the 2007-08 Ukrainian First League season.