In enzymology, a tryptophan 2-monooxygenase (EC 1.13.12.3) is an enzyme that catalyzes the chemical reaction

L-tryptophan + O₂ ${\displaystyle \rightleftharpoons }$ (indol-3-yl)acetamide + CO₂ + H₂O

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Thus, the two substrates of this enzyme are L-tryptophan and O₂, and its 3 products are (indol-3-yl)acetamide, CO₂, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O with incorporation of one atom of oxygen (internal monooxygenases o internal mixed-function oxidases). The systematic name of this enzyme class is L-tryptophan:oxygen 2-oxidoreductase (decarboxylating). This enzyme participates in tryptophan metabolism.