The Treason Act 1540 (32 Hen. 8. c. 25) was an Act of the Parliament of England. Its long title was "An Act declaring the Dissolution of the King's pretensed Marriage with the Lady Anne of Cleves."

The Act confirmed that the marriage between King Henry VIII and Anne of Cleves was annulled. They had married on 6 January 1540, but Henry had had the marriage annulled on 9 July of the same year. The Act also made it high treason for anyone "by word or deed, to accept, take, judge, or believe the said marriage to be good, or to procure or do any thing to the repeal of this act."

• Statutes at Large, vol. IV, Danby Pickering, Cambridge University Press, 1765.

• High treason in the United Kingdom
• Treason Act

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