In enzymology, a pyridoxine 4-dehydrogenase (EC 1.1.1.65) is an enzyme that catalyzes the chemical reaction

pyridoxine + NADP⁺ ${\displaystyle \rightleftharpoons }$ pyridoxal + NADPH + H⁺

Thus, the two substrates of this enzyme are pyridoxine and NADP⁺, whereas its 3 products are pyridoxal, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donors with NAD⁺ or NADP⁺ as acceptors. The systematic name of this enzyme class is pyridoxine:NADP⁺ 4-oxidoreductase. Other names in common use include pyridoxin dehydrogenase, pyridoxol dehydrogenase, and pyridoxine dehydrogenase. This enzyme participates in vitamin B₆ metabolism.