John Tyson may refer to:

• John J. Tyson (born 1947), professor in biological sciences at Virginia Tech
• John Baird Tyson (1928–2014), educator and explorer in the Himalayas
• John W. Tyson (died 1967), American businessman, founder of Tyson Foods
• John H. Tyson (born 1953), chairman and heir to Tyson Foods
• John M. Tyson (born 1953), North Carolina Court of Appeals Judge
• John R. Tyson (1856–1923), United States Representative from Alabama
• John R. Tyson (born 1990), chief financial officer at Tyson Foods
• John Tyson Wigan (1872–1952), British politician and army officer
• John C. Tyson (librarian) (1951–1995), African-American State Librarian of Virginia
• John C. Tyson (judge) (1926–2012), American jurist
• John A. Tyson (1873–1971), judge of the United States Tax Court

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