The Eastern Kentucky Colonels are the intercollegiate athletic teams of Eastern Kentucky University (EKU), located in Richmond, Kentucky, in intercollegiate sports as a member of the NCAA Division I ranks, primarily competing in the ASUN Conference since the 2021–22 academic year. Its football team competes in the United Athletic Conference (UAC), which starts play in 2023 as a football-only merger of the ASUN and the Western Athletic Conference (WAC). The Colonels previously competed in the Ohio Valley Conference (OVC) from 1948–49 to 2020–21.

The Colonels athletic program competes in NCAA Division I as a member of the ASUN Conference.^[2][3] The football team plays in the second level of Division I football, the Football Championship Subdivision (FCS). Before joining the ASUN in July 2021, EKU had spent the previous 73 years as a charter member^[4] of the Ohio Valley Conference (OVC).^[5] The EKU mascot is The Colonel, and the school colors are maroon and white. While the women's teams were formerly known as the Lady Colonels, the school now emphasizes that all teams are now Colonels.

The ASUN did not officially sponsor football until the 2022 season.^[6] In 2021, EKU football competed as a de facto associate member of the Western Athletic Conference (WAC), competing in a football partnership between the two leagues officially branded as the "ASUN–WAC Challenge".^[7] The ASUN–WAC alliance was renewed for the 2022 season, and the two conferences fully merged their football leagues after that season, announcing the football league's new branding of United Athletic Conference in April 2023.^[8]

NCAA

• Ohio Valley Conference (1948–2021)
• ASUN Conference (2021–present)

EKU's intercollegiate athletics teams are nicknamed the Colonels.^[9] Maroon has been the official color of Eastern athletics since the school was begun as the Eastern Kentucky State Normal School in 1906, and the school's official team nickname was the "Maroons" from the start of intercollegiate competition in 1909–10 until 1963, when then-president Robert Martin changed the nickname to the "Colonels".^[10]

A member of the ASUN Conference since July 2021, Eastern Kentucky University sponsors teams in eight men's and nine women's NCAA sanctioned sports.^[11]

EKU competes in 18 intercollegiate varsity sports: Men's sports include baseball, basketball, cross country, football, golf, tennis and track & field; while women's sports include basketball, beach volleyball, cross country, golf, soccer, softball, tennis, track & field and volleyball.

Historically, the Western Kentucky Hilltoppers and Lady Toppers served as the primary rival to EKU, especially in football. Their matchups, known as the "Battle of the Bluegrass," date back to 1914, with Western leading the overall series 34–47–3.^[15] With WKU's recent move up to the Football Bowl Subdivision, any future games between the two teams are uncertain.

Outside of Western, EKU has maintained rivalries with the only remaining in-state OVC member, Morehead State, and Murray State, which left the OVC in 2022 for the Missouri Valley Conference.