In enzymology, a diferric-transferrin reductase (EC 1.16.1.2) is an enzyme that catalyzes the chemical reaction

transferrin[Fe(II)]2 + NAD⁺ + H⁺ ${\displaystyle \rightleftharpoons }$ transferrin[Fe(III)]2 + NADH

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The 3 substrates of this enzyme are [[transferrin[Fe(II)]2]], NAD⁺, and H⁺, whereas its two products are [[transferrin[Fe(III)]2]] and NADH.

This enzyme belongs to the family of oxidoreductases, specifically those oxidizing metal ion with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is transferrin[Fe(II)]2:NAD+ oxidoreductase. Other names in common use include diferric transferrin reductase, NADH diferric transferrin reductase, and transferrin reductase. This enzyme participates in porphyrin and chlorophyll metabolism.

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1L5T.