The women's road time trial cycling events at the 2012 Summer Paralympics took place on 5 September at Brands Hatch.

Cyclists are given a classification depending on the type and extent of their disability. The classification system allows cyclists to compete against others with a similar level of function. The class number indicates the severity of impairment with "1" being most impaired.

Cycling classes are:^[1]

• B: Blind and visually impaired cyclists use a Tandem bicycle with a sighted pilot on the front.
• H 1–4: Cyclists with an impairment that affects their legs use a handcycle.
• T 1–2: Cyclists with an impairment that affects their balance use a tricycle.
• C 1–5: Cyclists with an impairment that affects their legs, arms and/or trunk, but are capable of using a standard bicycle.