The 20th North Carolina Infantry Regiment was an infantry regiment in the Confederate States Army. It was part of the Army of Northern Virginia for most of the war.

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20th North Carolina Infantry Regiment
Flag of North Carolina adopted on June 22, 1861
Active	July 1861 to April 1865
Country	Confederate States of America
Allegiance	North Carolina
Branch	Confederate States Army
Type	Regiment
Role	Infantry
Engagements	Peninsula Campaign Battle of Chancellorsville Battle of Gettysburg Overland Campaign Valley Campaigns of 1864 Appomattox Campaign

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The regiment was organized at Smithfield and Fort Caswell, North Carolina, in June, 1861. Its companies were recruited from the counties of Brunswick, Columbus, Cabarrus, Duplin, and Sampson counties. Alfred Iverson, Jr. was the regiment's first colonel, with Frank Faison as lieutenant colonel, and William H. Toon as major. It was first assigned to garrison duties in the coastal areas of North Carolina before being transferred to Samuel Garland, Jr.'s brigade, D. H. Hill's division of the Army of Northern Virginia in June 1862. It fought in the Peninsula Campaign and Maryland Campaign. Following Garland's mortal wounding in the Battle of South Mountain, Iverson was promoted to brigadier general and took command of the brigade.^[1]

In the spring of 1863, Captain Thomas F. Toon was promoted to colonel of the regiment. In the Battle of Chancellorsville, he was wounded, so Lieutenant Colonel Nelson Slough commanded the regiment in the Battle of Gettysburg. T. F. Toon recovered in time to return to command for the Overland Campaign. The regiment was detached with the rest of the Second Corps to the Shenandoah Valley for the summer and fall of 1864.

The regiment returned to the rest of the Army of Northern Virginia in March 1865 and participated in the Appomattox Campaign. It surrendered at Appomattox Courthouse on April 9, with four officers and 71 enlisted men remaining in the regiment.

• List of North Carolina Confederate Civil War units