In enzymology, a 2-chlorobenzoate 1,2-dioxygenase (EC 1.14.12.13) is an enzyme that catalyzes the chemical reaction

2-chlorobenzoate + NADH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ catechol + chloride + NAD⁺ + CO₂

The 4 substrates of this enzyme are 2-chlorobenzoate, NADH, H⁺, and O₂, whereas its 4 products are catechol, chloride, NAD⁺, and CO₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. The systematic name of this enzyme class is 2-chlorobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dechlorinating, decarboxylating). This enzyme is also called 2-halobenzoate 1,2-dioxygenase. This enzyme participates in benzoate degradation via coa ligation. It employs one cofactor, iron.