In enzymology, a 1,5-anhydro-D-fructose reductase (EC 1.1.1.263) is an enzyme that catalyzes the chemical reaction

1,5-anhydro-D-glucitol + NADP⁺ ${\displaystyle \rightleftharpoons }$ 1,5-anhydro-D-fructose + NADPH + H⁺

Thus, the two substrates of this enzyme are 1,5-anhydro-D-glucitol and NADP⁺, whereas its 3 products are 1,5-anhydro-D-fructose, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 1,5-anhydro-D-glucitol:NADP⁺ oxidoreductase.

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2GLX.